Fig. 2

Alignment of the deduced amino acid sequences of the Sol g 4.1 protein with other Solenopsis 2 and 4 venom proteins from S. invicta, S. geminata, S. saevissima, S. xyloni and S. richteri: conserved (red letters, green region), identical (yellow region), and groups of similar (turquois region) or non-similar (black letters, no color region) residues are shown. The end of the signal sequence is indicated by a blue triangle (). Alignment of the six cysteines (red stars) between all Solenopsis 2 and 4 genes and the alignment of the seventh cysteine in the Sol 2 genes (pink star). Residues lining the interior face of the Sol g 4.1 protein are indicated by x. The sequences were submitted to GenBank with the following accession numbers: Solenopsis 2 proteins: P35775 for Sol i 2, P35776 for Sol r 2, ABC58726 for Sol s 2, ALM98859 for Sol × 2, AAY32928 for Sol i 2q and AAY32926 for Sol g 2q; and Solenopsis 4 proteins: AAC97369 for Sol i 4.01, AAC97370 for Sol i 4.02, AAF65312 for Sol g 4.01, AAF65313 for Sol g 4.02, AAY32927 for Sol g 4q and AAY32929 for Sol i 4q