Fig. 3

Immunoblot detection of recombinant LlPLD1 from L. laeta venom by heterophilic antibodies of groups 1 and 2. a Immunoblot detection of recombinant protein rLlPLD1 (5 μg) with purified IgGs (1 μg/mL) of sera from groups 1 and 2. Positive control comprised mAb anti-rLlPLD1-7E4-D2 (1:50,000 dilution) and negative control was pre-immune mouse serum (1:1000 dilution). b Immunoprecipitation (IP) of L. laeta venom with antibodies from groups 1 and 2 sera, and detection by (upper panel) immunoblot with rabbit polyclonal L. laeta antivenom serum (1:10,000 dilution) or (below panel) anti-rLlPLD1 monoclonal antibody 7E4-D2 (1:50,000 dilution). Mouse L. laeta antivenom serum, pre-immune mouse serum, and unrelated antibody anti-BSA were used as IP control