Fig. 2From: Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extractsFractionation of SPE 25% and biological assay of its fractions. a RP-HPLC purification of 25% SPE fraction, in a C18 column, elution by 0 to 80% acetonitrile containing 0.1% TFA in water. In the arrow, the proinflammatory peak, named p3. b Leukocytes count on the cremaster muscle microcirculation 2 h after p3 injection. * p < 0.05Back to article page